Nuclear envelope formation around metaphase chromosomes: chromosome decondensation and nuclear envelope reconstitution during mitosis
Zatsepina, O.V.; Polyakov VYu; Chentsov YuS
European Journal of Cell Biology 26(2): 277-283
1982
ISSN/ISBN: 0171-9335 PMID: 7067705 Document Number: 190824
The hypothesis of a possible correlation between reconstitution of the nuclear envelope and decondensation of the chromosomes has been experimentally examined. In the presence of colcemid, which delays C-metaphase chromosome decondensation for several hours, compared to normal mitosis, nuclear envelope formation is also significantly delayed. On the contrary, the treatment of the cells with a hypotonic solution, resulting in some swelling of metaphase chromosomes, induces the establishment of contacts between these chromosomes and membranes and the formation of an envelope around them. A new method, by which micronuclei are formed under hypotonic treatment, is discussed.