The vector containing a signal for specific degradation of chimeric proteins. Synthesis of [Leu5]enkephalin using enteropeptidase
Dobrynin, V.N.; Boldyreva, E.F.; Filippov, S.A.; Chuvpilo, S.A.; Korobko, V.G.
Bioorganicheskaia Khimiia 13(1): 119-121
1987
ISSN/ISBN: 0132-3423 PMID: 3551962 Document Number: 303599
A plasmid vector (pEK1) coding, in framework of beta-galactosidase gene, for the amino acid sequence (Asp)4Lys which is recognized by bovine enteropeptidase has been constructed. Using this vector and chemically synthesized DNA coding for the [Leu5]-enkephalin, a plasmid (pEK-ENK) has been obtained in which the beta-galactosidase gene is fused, through the enteropeptidase linker, with the gene for [Leu5]enkephalin. The chimeric protein produced by expression of this plasmid has been isolated and then cleaved by the enteropeptidase to give [Leu5]enkephalin with the yield 74%.