Determination of lipid peroxidation products using thiobarbituric acid in anaerobic states
Kostiuk, V.A.; Potapovich, A.I.
Voprosy Meditsinskoi Khimii 33(3): 115-118
1987
ISSN/ISBN: 0042-8809 PMID: 3630005 Document Number: 301108
Products of lipid peroxidation were estimated in aerobic and anaerobic conditions using thiobarbituric acid (TBA) test. The main amount of malonic dialdehyde estimated in tissue homogenate in presence of oxygen was found to develop during the analytical procedure as a result of lipid peroxidation. At the same time, content of lipid peroxidation primary products did not correlate with amount of malonic dialdehyde in the sample studied. This suggests that TBA analysis may be carried out only under anaerobic conditions.