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The effect of delayed harvesting as well as freezing and thawing on biological properties of Newcastle disease virus

Trybała, E.

Acta Microbiologica Polonica 36(4): 287-292

1987

ISSN/ISBN: 0137-1320
PMID: 2457293
Document Number: 300878
Delayed harvest of Newcastle disease virus (NDV) from eggs was less infective (as expressed by its lower infectious titer and a higher number of virus particles per EID50) and more resistant to a temperature of 50.degree. C than the early harvest. On the other hand, little or no effect of delayed harvesting was found on neuraminidase, erythrocyte-fusing and immunogenic properties. Moreover, haemolytic activity of NDV was moderately enhanced when its harvesting from de-embryonated egg was delayed. Treatment of a fresh NDV preparation with repeated freezing and thawing cycles also caused a marked reduction in virus infectivity and induction of its haemolytic activity.

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