Freezing-thawing damages plasma membrane integrity of goat spermatozoa
Zhang, X.-g.; Zhao, Y.-j.; Li, Z.-q.
Zhonghua Nan Ke Xue 13(5): 403-406
2007
ISSN/ISBN: 1009-3591 PMID: 17569253 Document Number: 613084
To evaluate the plasma membrane integrity and morphology of fresh and frozen goat spermatozoa. The ejaculates of three male goats were obtained by the artificial vagina method of collection and the rates of sperm abnormality and acrosome integrity were detected after freezing-thawing processing. The plasma membrane integrity of the fresh and frozen-thawed goat spermatozoa was evaluated with a combination of fluorescent probes, carboxyfluorescein diacetate and propidium iodide. The freezing-thawing process significantly influenced the viability and integrity of the spermatozoa ([74.43 +/- 13.78]% vs. [46.25 +/- 2.69]%; [64.26 +/- 7.03]% vs. [6.27 +/- 2.90]%, P < 0.01). The results showed differences in acrosome integrity rate between the fresh and frozen samples ([80.77 +/- 10.70]% vs. [58.42 +/- 18.05]% , P < 0.05). The freezing-thawing process significantly reduces sperm viability and acrosome integrity and seriously damages the plasma membrane integrity.