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Effect of freezing and thawing on the structure and function of cytochrome oxidase

Rozanova, E.D.; Moiseev, V.A.; Naumenko, E.I.

Ukrainskii Biokhimicheskii Zhurnal 57(1): 61-64

1985

ISSN/ISBN: 0201-8470
PMID: 2983467
Document Number: 262467
The effect of freezing-thawing on cytochrome oxidase from cattle heart was studied. The enzyme was characterized for its activity, absorption spectra, temperature perturbation differential spectra and conformational transitions. A decrease in the activity and conformational changes depend on the composition of the buffer system and the presence of potassium and sodium chlorides in the solution.

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