Determination of encapsulation efficiency and release test of MTX polyphase liposome
Qi, H.; Li, H.Q.; Su, D.S.; Gu, X.Q.
Yao Xue Xue Bao 22(1): 48-52
1987
ISSN/ISBN: 0513-4870 PMID: 3604692 Document Number: 300750
With reference of the literature, a quick, acurate and simple method was developed for the determination of encapsulation efficiency in polyphase liposome (PL), based on the separation of the free drug molecules from PL on a minicolumn of sephadex G-25. It was observed that 22% of the total amount of MTX in the system had been entrapped in PL. In release test, it was found that MTX could be passively released from the internal compartments of the particles, but could not be taken up by them, regardless of long term storage at room temperature or being heated at higher temperatures.