Isolation of lipofuscin from the bovine myocardium
Tatariunas, A.B.; Orlov, N.Ia.; Karnaukhov, V.N.
Biulleten' Eksperimental'noi Biologii i Meditsiny 103(5): 543-546
1987
ISSN/ISBN: 0365-9615 PMID: 3593920 Document Number: 293458
The paper describes the procedure of lipofuscin isolation from bovine myocardium with the use of 15-45% (weight/volume) linear gradient of sucrose density and differential centrifugation. Two coloured populations of lipofuscin granules with different density have been distinguished. Electron microscopic studies of the isolated lipofuscin have revealed the homogeneity of fractions and good maintenance of the granules. The electron microscopic, absorptive and luminescent spectral properties of lipofuscin preparations correspond to its properties in situ. The isolation of lipofuscin preparations suggests the possibility of studying the chemical composition and intramolecular structure of the granules.