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Purification and characterization of glutathione transferase of human thyroid

Del Boccio, G.; Di Ilio, C.; Casalone, E.; Pennelli, A.; Aceto, A.; Sacchetta, P.; Federici, G.

Italian Journal of Biochemistry 36(1): 8-17

1987

ISSN/ISBN: 0021-2938
PMID: 3583687
Document Number: 289879
An anionic (pI 4.6) isoenzyme of glutathione transferase was purified to homogeneity from human thyroid by affinity chromatography followed by isoelectric focusing. The content of enzyme was calculated to constitute about 0.2% of soluble proteins. The enzyme is formed by two identical subunits of 23,000 daltons approximately. The thyroid transferase did not catalyze the reduction of peroxides. Physical, catalytic and immunological analyses demonstrated extensive similarities between the thyroid transferase and the transferase from placenta, erythrocytes and breast. On the other hand, the thyroid transferase appears catalytically different from transferase 7-7, even if both cross-react with the antibodies raised against human placenta transferase.

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Dierickx, P.J.; Almar, M.M.; De Jonckheere, J.F. 1990: Glutathione transferase activity in some flagellates and amoebae, and purification of the soluble glutathione transferases from Acanthamoeba Biochemistry International 22(4): 593-600
Hall, A.G.; Matheson, E.; Hickson, I.D.; Foster, S.A.; Hogarth, L. 1994: Purification of an alpha class glutathione S-transferase from melphalan-resistant Chinese hamster ovary cells and demonstration of its ability to catalyze melphalan-glutathione adduct formation Cancer Research 54(13): 3369-3372
Szarka, C.E.; Pfeiffer, G.R.; Hum, S.T.; Everley, L.C.; Balshem, A.M.; Moore, D.F.; Litwin, S.; Goosenberg, E.B.; Frucht, H.; Engstrom, P.F. 1995: Glutathione S-transferase activity and glutathione S-transferase mu expression in subjects with risk for colorectal cancer Cancer Research 55(13): 2789-2793
Sherman, M.; Titmuss, S.; Kirsch, R.E. 1983: Glutathione S-transferase in human organs Biochemistry International 6(1): 109-118
Sasano, H.; Miuazaki, S.; Shiga, K.; Goukon, Y.; Nishihira, T.; Nagura, H. 1993: Glutathione S-transferase in human esophageal carcinoma Anticancer Research 13(2): 363-368
Leyland-Jones, B.R.; Townsend, A.J.; Tu, C.P.; Cowan, K.H.; Goldsmith, M.E. 1991: Antineoplastic drug sensitivity of human MCF-7 breast cancer cells stably transfected with a human alpha class glutathione S-transferase gene Cancer Research 51(2): 587-594
Moscow, J.A.; Fairchild, C.R.; Madden, M.J.; Ransom, D.T.; Wieand, H.S.; O'Brien, E.E.; Poplack, D.G.; Cossman, J.; Myers, C.E.; Cowan, K.H. 1989: Expression of anionic glutathione-S-transferase and P-glycoprotein genes in human tissues and tumors Cancer Research 49(6): 1422-1428
Tew, K.D.; Woodworth, A.; Stearns, M.E. 1986: Relationship of glutathione depletion and inhibition of glutathione-S-transferase activity to the antimitotic properties of estramustine Cancer Treatment Reports 70(6): 715-720
Gupta, M.P.; Dogra, S.C.; Khanduja, K.L.; Sharma, R.R. 1983: Effect of vitamin A deficiency on the level of glutathione and glutathione S-transferase activity in rat colon and liver Acta Vitaminologica et Enzymologica 5(3): 141-145
Adams, K.J.; Carmichael, J.; Wolf, C.R. 1985: Altered mouse bone marrow glutathione and glutathione transferase levels in response to cytotoxins Cancer Research 45(4): 1669-1673
Caccuri, A.M.; Aceto, A.; Rosato, N.; Di Ilio, C.; Piemonte, F.; Federici, G. 1991: Intrinsic fluorescence quenching of glutathione transferase pi by glutathione binding Italian Journal of Biochemistry 40(5): 304-311
Polidoro, G.; Zulli, P.; Di Ilio, C.; Del Boccio, G.; Di Cola, D.; Federici, G. 1979: Preliminary study of the physical chemistry and catalytic properties of glutathione-S-transferase from human placenta Bollettino della Societa Italiana di Biologia Sperimentale 55(9): 840-843
Yilmaz, S.; Widersten, M.; Emahazion, T.; Mannervik, B. 1995: Generation of a Ni(II) binding site by introduction of a histidine cluster in the structure of human glutathione transferase A1-1 Protein Engineering 8(11): 1163-1169
Vaury, C.; Lainé, R.; Noguiez, P.; de Coppet, P.; Jaulin, C.; Praz, F.; Pompon, D.; Amor-Guéret, M. 1995: Human glutathione S-transferase M1 null genotype is associated with a high inducibility of cytochrome P450 1A1 gene transcription Cancer Research 55(23): 5520-5523
Shiratori, Y.; Soma, Y.; Maruyama, H.; Sato, S.; Takano, A.; Sato, K. 1987: Immunohistochemical detection of the placental form of glutathione S-transferase in dysplastic and neoplastic human uterine cervix lesions Cancer Research 47(24 Part 1): 6806-6809
Cai, L.-Q.; Li, M.; Lu, S.; Wang, H.-B.; Wang, Z.-H. 2004: Killing effect of adenovirus mediated fusion gene cytosine and deaminase uracil phosphoribosyl transferase directed by glutathione S-transferase P1 promoter on cisplatin-resistant ovarian cancer cells in vitro Zhonghua Fu Chan Ke Za Zhi 39(7): 478-481
Kalinina, E.V.; Novichkova, M.D.; Shcherbak, N.P.; Saprin, A.N. 2000: Inhibitory effect of progesterone P1-1 on glutathione-s-transferase and its antiproliferative effect on human erythroleukemia K562 cells Voprosy Onkologii 46(1): 68-73
Hu, M.; Pollock, R.E.; Nicolson, G.L. 1997: Purification and characterization of human lung fibroblast motility-stimulating factor for human soft tissue sarcoma cells: identification as an NH2-terminal fragment of human fibronectin Cancer Research 57(16): 3577-3584
Wang, Y.Y.; Teicher, B.A.; Shea, T.C.; Holden, S.A.; Rosbe, K.W.; al-Achi, A.; Henner, W.D. 1989: Cross-resistance and glutathione-S-transferase-pi levels among four human melanoma cell lines selected for alkylating agent resistance Cancer Research 49(22): 6185-6192
Hu, X.; Herzog, C.; Zimniak, P.; Singh, S.V. 1999: Differential protection against benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide-induced DNA damage in HepG2 cells stably transfected with allelic variants of pi class human glutathione S-transferase Cancer Research 59(10): 2358-2362