Antiviral activity of mutant interferons. a new approach to the study of structural-functional organization of interferons
Petrenko, V.A.; Tat'kov, S.I.; Sivolobova, G.F.; Boldyrev, A.N.; Kolokol'tsev, A.A.
Bioorganicheskaia Khimiia 13(2): 259-262
1987
ISSN/ISBN: 0132-3423 PMID: 3555509 Document Number: 287441
The developed approach to investing the structure-functional organization of interferon has been developed consisting in: 1) fusing genes of interferon and alpha-peptide of beta-galactosidase, the resultant protein having the interferon properties and being determined by the beta-galactosidase alpha-complementation test; 2) constructing mutant genes of interferon by the localized mutagenesis; 3) determining the mutant interferon activity; 4) deducing the amino acid sequence of mutant interferon by sequencing mutant genes; 5) analyzing structure-functional organization of interferon. In accordance with this approach, ten mutant interferons with up to 15 changes of amino acid substitutions are obtained and their antiviral activity is determined. The role of some amino acid residues in antiviral activity of interferon alpha 2 is revealed.