Release of polyoma virus from transformed human cells
Klenova, A.V.; Borodina, N.P.; Shevliagin, V.I.
Voprosy Onkologii 33(11): 57-61
1987
ISSN/ISBN: 0507-3758 PMID: 2825430 Document Number: 287031
Virus reproduction and activation were studied in human cells transformed with human polyoma virus. At the stage of spontaneous viral production, such methods of activation as somatic hybridization and DNA transfection were successful. Special methods of viral activation (treatment with mitomycin C and DEAE--dextran, and subsequent homologous DNA transfection) were required when viral production by cells ceased completely.
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