Representation of the leader region of Rous sarcoma virus genome RNA in double-stranded RNA produced in virus-transformed cells
Perevozchikov, A.P.; Sizonenko, G.I.
Molekuliarnaia Biologiia 21(5): 1179-1185
1987
ISSN/ISBN: 0026-8984 PMID: 2824985 Document Number: 292556
Restriction fragments of recombinant plasmids containing a proviral sequence of Rous sarcoma virus (RSV) were Southern hybridized with double-stranded (ds) RNA isolated from the cells transformed with RSV. Hybridization data show that the major subpopulation of dsRNA molecules is homologous to the 5'-end region of the viral genome including the leader sequence. We have analysed the RNAs of RSV-transformed cells by the Northern procedure hybridizing them with the proviral fragment containing double long terminal repeats. The results demonstrate that the 14-16S RNA fraction is enriched in sequences which are homologous to the proviral end regions. We consider this RNA fraction to be homologous to the 5'-terminal region of the viral genome and (or) to its antisense strand.