Interaction of immunoliposomes with avian myeloblastosis virus and virus transformed cells
Dhananjaya, K.V.; Antony, A.
Indian Journal of Experimental Biology 26(12): 965-968
1988
ISSN/ISBN: 0019-5189 PMID: 2855518 Document Number: 313943
Immunoliposomes were prepared using the antibody raised against avian myeloblastosis virus (AMV) major envelope glycoprotein, gp80. They were found to bind to polystyrene plates coated with AMV. The binding of immunoliposomes to AMV was analysed by scanning electron microscopy. Quantitative analysis revealed that immunoliposomes bind to virus to a greater extent than free liposomes or nonspecific immunoliposomes. The binding of immunoliposomes to virus-transformed cells was inhibited when the cells were preincubated with homologous antibody. Incubation at low temperature and pretreatment of cells with metabolic inhibitors decreased the uptake of immunoliposomes by virus transformed cells. The drug encapsulated in immunoliposomes was not significantly taken up by nontarget cells.