Changes in the mechano-chemical properties of actomyosin during desensitization
Kropacheva, I.V.; Korchagin, V.P.; Shelud'ko, N.S.
Biokhimiia 51(5): 834-839
1986
ISSN/ISBN: 0320-9725 PMID: 2939884 Document Number: 284357
The loss of Ca2+-sensitivity by natural actomyosin (desensitisation) after treatment with low ionic strength solutions results in marked deceleration of protein superprecipitation. This phenomenon is not due to the removal of minor proteins, since a similar effect was observed during "desensitisation" of synthetic actomyosin containing only myosin and actin. However, addition to desensitised actomyosin of tropomyosin, especially in combination with alpha-actinin markedly restores the initial parameters of superprecipitation and ATPase activity. It was assumed that desensitisation has a direct modifying influence on actomyosin, whose effect is weakened in the presence of tropomyosin and alpha-actinin.