Use of inversion spin transfer to monitor creatine kinase kinetics in rat skeletal muscle in vivo
Haseler, L.J.; Brooks, W.M.; Irving, M.G.; Bulliman, B.T.; Kuchel, P.W.; Doddrell, D.M.
Biochemistry International 12(4): 613-618
1986
ISSN/ISBN: 0158-5231 PMID: 3718523 Document Number: 281869
A simple multipulse sequence has been used to monitor creatine kinase kinetics in rat skeletal muscle in vivo. Using these procedures, the forward (ATP synthesis) and reverse fluxes (phosphocreatine synthesis) have been calculated to be 8.98 .+-. 0.6 and 10.7 .+-. 0.8 .mu.moles/g wet wt/s (n = 5) respectively. These results suggest that in resting skeletal muscle most of the .gamma.ATP observed in 31P NMR spectra is cytosolic and rapidly exchanging with phosphocreatine. The high flux rates reflect the high catalytic capacity of creatine kinase in skeletal muscle.