Production and isolation of aflatoxin M1 for toxicological studies
Hsieh, D.P.; Beltran, L.M.; Fukayama, M.Y.; Rice, D.W.; Wong, J.J.
Journal - Association of Official Analytical Chemists 69(3): 510-512
1986
ISSN/ISBN: 0004-5756 PMID: 3087945 Document Number: 277184
One hundred mg aflatoxin M1 was produced and purified for toxicological studies. Aspergillus flavus NRRL 3251 was cultured on rice to produce aflatoxins B1, B2, M1, and M2, B1 and B2 were separated from M1 and M2 by a normal phase low pressure liquid chromatography (LC) column. M1 was then separated from M2 by a reverse phase low pressure LC column. Recoveries of aflatoxins from the LC columns were about 90%. The purified M1 was confirmed by ultraviolet-visible spectrometry, mass spectrometry, nuclear magnetic resonance spectrometry, optical rotation, and its mutagenicity to Salmonella typhimurium TA98.