Differentiation potentials of clonal strains of bone marrow fibroblasts
Gerasimov, I.V.; Fridenshteĭn, A.I.; Chaĭlakhian, R.K.; Shishkova, V.V.
Biulleten' Eksperimental'noi Biologii i Meditsiny 101(6): 717-719
1986
ISSN/ISBN: 0365-9615 PMID: 3524701 Document Number: 275607
The clonal nature of CFUf-derived fibroblast colonies was proved by chromosomal analysis of individual colonies and single-colony-derived fibroblast strains using mixed cell cultures from male and female rabbits. CFUf progeny, forming colonies composed of more than 10(3) cells was capable of 20-30 cell doublings during subsequent passages. When transplanted in diffusion chambers, single-colony-derived fibroblast strains formed bone and cartilage simultaneously. Thus, CFUf or part of them can be regarded as bone marrow osteogenic stem cells.