Myeloid and erythroid hemopoiesis supported by human bone marrow fibroblasts in vitro
Cao, W.H.; Wang, Q.R.; Xu, Y.H.
Chinese Medical Journal 105(7): 544-548
1992
ISSN/ISBN: 0366-6999 PMID: 1451558 Document Number: 391730
The passages 0 and 3 purified human bone marrow fibroblast layers (FLs) were established by long-term liquid cultures. After 12-day coculture of stromal, cell-depleted marrow cell suspensions with passage 0 FLs, 68.33 +- 4.04% of the hemopoietic colonies adhered to FLs was myeloid in nature and the other 31.67 +- 4.04% was erythroid. There were still CFU-E (colony forming unit-erythroid), BFU-E (burst forming unit-erythroid), and CFU-GM (colony forming unit-granulocyte/macrophage) among the nonadherent cells. The media conditioned by passage 0 (F-0-CM) and passage 3 (F-3-CM) FLs stimulated the growth of myeloid and erythroid (BFU-E) colonies. From these data, it is concluded that both FLs and the media conditioned by fibroblasts can stimulate myeloid and erythroid hemopoiesis.