Analysis of macrophage functional activity during adhesion
Barsukov, A.A.; Zemskov, V.M.; Beznosenko, S.A.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 1: 3-8
1986
ISSN/ISBN: 0372-9311 PMID: 3705803 Document Number: 270629
Using the phenomenon of the formation of the active forms of oxygen by phagocytes in the process of adhesion, changes occurring in the cell membrane under the influence of sodium caseinate, macrophage migration inhibition factor, aggregated IgG and hydrolytic enzymes could be registered. Sodium caseinate inhibited chemiluminescence during the first 10 minutes in the adhesion of mouse peritoneal macrophages, human lung macrophages and adhesive marrow cells, while large doses of this preparation activated chemiluminescence at the latter stages of adhesion. Macrophage migration inhibition factor induced the rapid and prolonged formation of the active forms of oxygen in the process of macrophage adhesion, aggregated IgG decreased this cell function, and the subsequent phagocytosis of zymosan was accompanied by increased chemiluminescence. The experimental and practical possibilities of this phenomenon are discussed.