Hydrolytic stability of a covalent AMP-RNA ligase complex

Iuodka, B.A.; Markutskas, A.I.

Bioorganicheskaia Khimiia 11(6): 821-825

1985


ISSN/ISBN: 0132-3423
PMID: 2994684
Document Number: 252826
Behavior of the covalent [32P]- and [14C]AMP-RNA ligase complex under various conditions has been studied. The covalent structure is shown to be readily cleaved by acid and hydroxylamine and relatively stable to alkali and snake venom phosphodiesterase. Products of degradation of the AMP-RNA ligase and AMP-DNA ligase complexes were compared. The data obtained support the earlier assumption of a phosphoamide bond in the AMP-RNA ligase compound.

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