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Base modification and cloning efficiency of oligodeoxyribonucleotides synthesized by the phosphoramidite method: methyl versus cyanoethyl phosphorus protection

Urdea, M.S.; Ku, L.; Horn, T.; Gee, Y.G.; Warner, B.D.

Nucleic Acids Symposium Series 16: 257-260

1985

ISSN/ISBN: 0261-3166
PMID: 4088879
Document Number: 245474
We have found large oligodeoxyribonucleotides (50-120 bases) synthesized with N,N-dialkylmethylphosphoramidites to have considerably lower cloning efficiencies than biological DNA. As evidenced by HPLC analysis, these large fragments contain as much as 30% thymidine conversion to 3-methylthymidine. If cyanoethyl- instead of methyl-phosphorous protection is employed, as anticipated, no thymidine methylation is noted. More importantly, large fragments produced by N,N-diisopropylcyanoethyl-phosphoramidite coupling show cloning efficiencies indistinguishable from biological DNA.

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Mandecki, W.; Shallcross, M.A.; Kavanaugh, T.J. 1990: Simple biological assay for the error rate upon cloning of synthetic oligodeoxyribonucleotides Biotechniques 9(1): 56-59
Han, S.-f.; Deng, R.-l.; Xu, H.-r.; Cao, Y.-f.; Wang, X.-y.; Xiao, K. 2007: Photosynthesis and active-oxygen-scavenging enzyme activities in rice varieties with different phosphorus efficiency under phosphorus stress Ying Yong Sheng Tai Xue Bao 18(11): 2462-2467
Kobylańska, A.; Pluskota, E.; Swiatkowska, M.; Wójcik, M.; Cierniewska-Cieślak, A.; Krakowiak, A.; Boczkowska, M.; Pawłowska, Z.; Okruszek, A.; Koziołkiewicz, M.; Cierniewski, C.S.; Stec, W.J. 1999: Inhibition of plasminogen activator inhibitor release in endothelial cell cultures by antisense oligodeoxyribonucleotides with a 5'-end lipophilic modification Acta Biochimica Polonica 46(3): 679-691
Zhang, S.R.; Xu, R.M.; Jin, B.Y.; Wu, Y.L.; Han, C. 1996: Protection of dl-(15R)-15 methyl-PGE2 methyl ester (PG6E) on experimental ulcers in rats Yao Xue Xue Bao 31(10): 746-750
Gait, M.J.; Popov, S.G.; Singh, M.; Titmas, R.C. 1980: Rapid synthesis of oligodeoxyribonucleotides. V. Further studies in solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates Nucleic Acids Symposium Series 7: 243-257
Priĭmak, A.A.; Sokolova, G.B.; Bel'kind, A.M.; Khaĭkin, B.I.; Braude, V.I. 1987: Aldozone--a new derivative of isoniazid synthesized using a monomeric hydrocarbon matrix as the base Problemy Tuberkuleza 1: 53-57
Naruto, M. 1982: Synthesis of oligodeoxyribonucleotides by solid phase phosphotriester method on a reduced scale; preparation of oligonucleotides for improved promoter sequence Nucleic Acids Symposium Series 11: 77-80
Bittle, J.L.; Houghten, R.A.; Alexander, H.; Shinnick, T.M.; Sutcliffe, J.G.; Lerner, R.A.; Rowlands, D.J.; Brown, F. 1982: Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence Nature 298(5869): 30-33
Pawar, G.B.; Todai, N.K.; Jaffar, M.B. 1978: A useful modification to the colorimetric assay of inorganic phosphorus and alkaline phosphatase in serum Clinical Chemistry 24(10): 1847-1848
Gruber, C.E. 1992: High-efficiency cDNA cloning: a comparison of electroporation and in vitro packaging Biotechniques 12(6): 804
Schaefer, K.E.; Carey, C.R.; Dougherty, J.H.; Morgan, C.; Messier, A.A. 1979: Effect of intermittent exposure to 3% CO2 on respiration, acid-base balance, and calcium-phosphorus metabolism Undersea Biomedical Research 6(Suppl): S115-S134
Miyoshi, K.; Itakura, K. 1980: Solid-phase synthesis of polynucleotides: V. Synthesis of oligodeoxyribonucleotides by the phosphomonotriazolide method Nucleic Acids Symposium Series 7: 281-291
Hamuro, Y.; Shino, A.; Suzuoki, Z. 1970: Acute induction of soft tissue calcification with transient hyperphosphatemia in the KK mouse by modification in dietary contents of calcium, phosphorus, and magnesium Journal of Nutrition 100(4): 404-412
Huang, A.-C.; Hsu, S.-C.; Kuo, C.-L.; Liao, C.-L.; Lai, K.-C.; Lin, T.-P.; Wu, S.-H.; Lu, H.-F.; Tang, N.-Y.; Yang, J.-S.; Chung, J.-G. 2009: Involvement of matrix metalloproteinases in the inhibition of cell invasion and migration through the inhibition of NF-[kappa]B by the new synthesized ethyl 2-[N-p-chlorobenzyl-(2'-methyl)]anilino-4-oxo-4,5-dihydrofuran-3-carboxylate (JOTO1007) in human cervical cancer Ca ski cells In Vivo 23(4): 613-619
Sharov, V.G.; Saks, V.A.; Kupriyanov, V.V.; Lakomkin, V.L.; Kapelko, V.I.; Steinschneider AYa; Javadov, S.A. 1987: Protection of ischemic myocardium by exogenous phosphocreatine. I. Morphologic and phosphorus 31-nuclear magnetic resonance studies Journal of Thoracic and Cardiovascular Surgery 94(5): 749-761
Malizia, E.; Pietropaoli, P.; Signore, L. 1970: Use of tris-hydroxy-methyl-amino methane in acid-base changes in resuscitation La Clinica Terapeutica 53(5): 405-429
Lazaridis, K.; Dang, H.; Talal, N. 1993: Human kappa chain expression in a lambda phage vector: methods of isolating amplified cDNA affects cloning efficiency Human Antibodies and Hybridomas 4(3): 104-106
Lange, K.W. 1989: Modification by apomorphine of the circling behaviour in the rat due to unilateral intranigral injection of 1-methyl-4-phenylpyridine (MPP+) Journal de Toxicologie Clinique et Experimentale 9(1): 21-25
Mackay, S.P.; Comoe, L.; Desoize, B.; Duval, O.; Jardillier, J.C.; Waigh, R.D. 1998: The effect of 12-alkoxy modification on the in vitro antileukaemic activity of N-methyl 2,3,8,9-tetramethoxybenzo[c]phenanthridinium salts Anti-Cancer Drug Design 13(7): 797-813
Kaĭdin, D.A. 1973: Modification of a rapid method of analysis of total blood serum lipids by Huergo's method Laboratornoe Delo 12: 750-751