Isolation and quantitative determination of apoprotein C-11 in the blood using rocket immunoelectrophoresis

Shcherbakova, I.A.; Petrova, N.V.; Metel'skaia, V.A.; Nikitina, N.A.; Chernysheva, N.P.

Voprosy Meditsinskoi Khimii 30(6): 82-88

1984


ISSN/ISBN: 0042-8809
PMID: 6528541
Document Number: 240950
Apoprotein C-II was isolated from human blood plasma by means of column chromatography-gel filtration and DEAE ion exchange chromatography by modified method of Brown et al (1969). Production of antiserum to apoprotein C-II as well as a procedure for rocket immunoelectrophoresis are described for estimation of the apoprotein in blood plasma of men and women of various age, exhibiting different lipid spectra. Content of the apoprotein C-II in blood plasma, estimated by the immunoelectrophoretic procedure, was higher in men as compared with women and correlated with concentration of triglycerides in blood plasma.

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