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Cloning of apoprotein AI gene and observation on DNA polymorphism of apoprotein AI/CIII gene-loci in genomic library from a liver of Chinese

Xu, H.J.; Zhou, H.Y.; Hu, S.X.; Pan, X.R.

Chinese Medical Journal 102(3): 169-173

1989

ISSN/ISBN: 0366-6999
PMID: 2569382
Document Number: 333932
For the research on the structure and function of the HDL apoproteins, we have successfully constructed a complete genomic library, in self-prepared EMBL3 .lambda. vector and the packaging extracts of lysogenic strains BHB2688, BHB2690, from a Chinese fetal liver, which will be widely used in the research on the structure and function of apoproteins. In this study, a DNA sequence polymorphism, revealed by digestion of human DNA with the restriction endonuclease Sst1 and hybridization with an aproprotein AI cDNA probe, has been shown to the located at apoAI / CIII gene-loci. Three gene related fragments (5.7Kb, 4.0Kb and 3.0Kb) were detected. The 5.7Kb fragment is common and the polymorphism is demonstrated by the presence of either a 4.0Kb fragment (S1 allele) or the 3.0Kb fragment (S2 allele). Individuals were genotyped S1S1 or S1S2. Our result showed that the frequency of genotype S1S2 was higher in hypertriglyceridemic subjects than that in normolipidemic subjects.

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