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Construction of gene library of attenuated liver hepatitis A vaccine (H2 strain)

Chen, Y.; Yang, N.; Hong, Y.

Zhonghua Yi Xue Za Zhi 76(5): 342-344

1996

ISSN/ISBN: 0376-2491
PMID: 9206195
Document Number: 468137
To determine the complete gene library of attenuated live hepatitis A vaccine (H2 strain) from cloned cDNA by application of reverse transcription (RT) and polymerase chain reaction (PCR) technique. Attenuated HAV H2 strain that was selected and studied in China was proven attenuated for human beings and proved to be effective in preventing hepatitis A. Ten overlapping cDNA clones were obtained in addition to spanned the entire genome. The restriction enzyme mapping was also determined. The gene library will benefit for the sequence of HAV cDNA and study on attenuation.

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