Use of a TLC method for the simultaneous determination of an enzyme complex of nucleic acid metabolism during the isolation of polynucleotide phosphorylase
Pupkova, V.I.; Raspopina, G.I.
Prikladnaia Biokhimiia i Mikrobiologiia 20(3): 428-431
1984
ISSN/ISBN: 0555-1099 PMID: 6205391 Document Number: 230057
For the simultaneous determining of several enzymes of nucleic acid metabolism during polynucleotide phosphorylase isolation TLC was used. It was found that using TLC one can simultaneously detect six and more enzymes, e. g. polynucleotide phosphorylase, 5'-nucleotidase, exoribonuclease together with nucleosidediphosphatase, desaminase etc. The method is simple and accessible.