DNA-phospholipid interaction. 31P-NMR study
Viktorov, A.V.; Grepachevskiĭ, A.A.; Bergel'son, L.D.
Bioorganicheskaia Khimiia 10(7): 935-939
1984
ISSN/ISBN: 0132-3423 PMID: 6497914 Document Number: 227831
The phage T7 DNA complexes with various phospholipids (PL) were studied by 31P NMR at PL/nucleotide molar ratio of 2 : 1. Using a phosphatidylcholine thion analogue, the contributions of PL and DNA into the 31P NMR spectrum of the complex were estimated. It was found that PL-DNA interaction results in partial immobilizing ability of PL depends on their structure, increasing in the following row: phosphatidylcholine-phosphatidylethanolamine less than sphingomyelin less than or equal to ternary mixture of these PL. The data obtained are indicative of nonequivalent binding of DNA with various PL species.