A comparative study of the utilization of ether- and ester-linked phospholipid-containing liposomes by J774.E1 macrophage cell-line infected with Leishmania mexicana mexicana amastigotes
Hrcková, G.; Shah, A.; Hart, D.T.; Halton, D.W.
Folia Parasitologica 41(3): 161-167
1994
ISSN/ISBN: 0015-5683 PMID: 7883247 Document Number: 437898
The macrophage cell-line J774.E1 infected with Leishmania mexicana mexicana was used to investigate the uptake of liposomes, which differed in their bulk phospholipid:ester- or ether-analogue of phosphatidylcholine (PC). The receptor-mediated uptake of both species of liposomes, containing native or acetylated LDL (low density lipoproteins) as ligands was also evaluated. Uninfected and infected J774.E1 accumulated more ester- and ether-liposomes separately than the mixed type (50:50 ester/ether). The utilization was significantly enhanced when both types of liposomes contained native LDL. The highest uptake was recorded for liposomes bearing acetylated LDL by infected J774.E1. Accumulation of ester- and ether-liposomes with the same ligand was not markedly affected by the different chemical nature of PC. Ether-liposomes alone possessed some activity against Leishmania mexicana mexicana amastigotes.