Nuclear DNAase of the yeast Candida tropicalis
Davydova, M.N.; Kupriianova, F.G.; Vinter, V.G.
Biokhimiia 49(2): 310-315
1984
ISSN/ISBN: 0320-9725 PMID: 6370318 Document Number: 224958
Using stepwise extraction of chromatin from C. tropicalis by NaCl (0.1-1.0 M) the proteins dissociated by 0.3 and 0.6 M NaCl (fractions 0.3 and 0.6) possessing the DNase activity were obtained. These DNase are activated by Mg2+ and cause preferential hydrolysis of heat-denaturated DNA. Fraction 0.3 DNase has a maximum at neutral values of pH (.apprx. 7.0) and causes endonucleolytic hydrolysis of DNA. Fraction 0.6 DNase causes exonucleolytic hydrolysis of DNA but a maximum at alkaline pH (8.0).