Moraxella bovis hemolysin
Ostle, A.G.; Rosenbusch, R.F.
American Journal of Veterinary Research 45(9): 1848-1851
1984
ISSN/ISBN: 0002-9645 PMID: 6497144 Document Number: 223856
M. bovis hemolysin was readily filterable through polycarbonate membrane filters, but not through nitrocellulose filters. The hemolysin was filterable through polycarbonate filters with pore diameters of .gtoreq. 0.015 .mu.m (APD). Of the hemolytic activity of cell-free filtrates, 74% could be pelleted by ultracentrifugation at 100,000 .times. g for 2.5 h. Hemolytic activity could be demonstrated in preparations of outer membrane fragments isolated from log-phase cultures. Hemolysin in M. bovis broth cultures reached a maximum concentration in late log-arithmic phase (4.5 h after inoculation) and declined thereafter. Hemolysin was inactivated by heat, trypsin, formalin and lyophilization. [Implications with respect to the development of an antihemolytic factor in the protection of cattle from M. bovis associated keratoconjunctivitis.].