Purification of V. cholerae El Tor hemolysin
Chaicumpa, W.; Atthasishtha, N.
Southeast Asian Journal of Tropical Medicine and Public Health 10(1): 18-22
1979
ISSN/ISBN: 0125-1562 PMID: 483015 Document Number: 148249
The hemolysin purification by a procedure which is modified from that of Watanabe and Seaman is described. It resulted in a 2% recovery of the hemolytic activity. The final preparation was called pure hemolysin since it gave only a single precipitin band with the rabbit antiserum to crude hemolysin in immunoelectrophoresis.
Document emailed within 1 workday
Related Documents
Chaicumpa, W.; Atthasishtha, N. 1979: The study of intestinal immunity against V. cholerae: purification of V. cholerae El Tor haemagglutinin and the protective role of its antibody in experimental cholera Southeast Asian Journal of Tropical Medicine and Public Health 10(1): 73-80Arellano Galindo, Jé.; Rodriquez Angeles, Mía.Guadalupe.; Guadarrama, N.Valázquez.; Esteban, E.Santos.; Cerezo, S.Giono. 2007: Evaluation of in vivo and in vitro biological activity of a Vibrio cholerae 01 hemolysin Clinical and Investigative Medicine 30(6): E250-E256
Telesmanich, I.R.; Men'shikov, E.A.; Kurbatova, E.M.; Goncharenko, E.V. 2010: Role of galactose-specific receptor--lectin in bactericidal activity of hemolysin of Vibrio cholerae non O1/O139 Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 1: 10-14
Guard, R.W.; Brigden, M.; Desmarchelier, P. 1980: Fulminating systemic infection caused by Vibrio cholerae species which does not agglutinate with 0-1 V. cholerae antiserum Medical Journal of Australia 1(13): 659-661
Modak, S.; Modak, M.J.; Venkataraman, A. 1970: Mechanism of action of berberine on Vibrio cholerae and vibrio cholerae biotype eltor Indian Journal of Medical Research 58(11): 1510-1522
Ostle, A.G.; Rosenbusch, R.F. 1984: Moraxella bovis hemolysin American Journal of Veterinary Research 45(9): 1848-1851
Le Bouffant, L.; Henin, J.P.; Martin, J.C.; Da Niel, H. 1972: Experimental study of pulmonary purification. Action of the dust covering on the purification capacity. Application to humans Lille Medical: Journal de la Faculte de Medecine et de Pharmacie de l'Universite de Lille 17(8): 1091-1101
Gracheva, M.P.; Shkodich, P.E.; Tregubov, B.A.; Tikhomirov, I.P.; Filiukova, T.V. 1974: Use of ozone for final purification of industrial effluents following biological purification Gigiena i Sanitariia 12: 90-91
Sandhu, T.S.; White, F.H. 1977: Production and characterization of Moraxella bovis hemolysin American Journal of Veterinary Research 38(6): 883-885
Griswold, D.E.; Heppner, G.H.; Calabresi, P. 1975: Stimulation of hemolysin plaque-forming cells by idoxuridine Cancer Research 35(1): 88-92
Dobardzic, R.; Sonea, S.; Côté, J.R. 1970: Effect of double lysogenization on elaboration of beta-hemolysin and staphylokinase Revue Canadienne de Biologie 29(1): 1-6
Palkina, N.A.; Raskin, B.M.; Denisova, S.V.; Kushnarev, V.M.; Levadnaia, T.B. 1977: Nutrient medium for obtaining alpha hemolysin from Escherichia coli Laboratornoe Delo 2: 106-108
Sergeeva, T.I.; Dolgikh, M.S.; Blagoveshchenskiĭ, V.A. 1981: Immunogenic properties of Clostridium perfringens type a anatheta-hemolysin Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 11: 71-75
Sakurai, J.; Honda, T.; Jinguji, H.; Miwatani, T. 1975: Thermostable direct hemolysin of Vibrio parahaemolyticus. (VI) its mechanism of action on FL cells Nihon Saikingaku Zasshi. Japanese Journal of Bacteriology 30(1): 232
Honda, T. 1977: Lethal activities of the thermostable direct hemolysin produced by Vibrio parahaemolyticus Nihon Saikingaku Zasshi. Japanese Journal of Bacteriology 32(6): 777-784
Badin, J.; Lajarrige, S. 1972: Inhibition of a macromolecular hemolysin, streptolysin O, with some hemolytic fatty acids. Mechanism of this action Nouvelle Revue Francaise d'Hematologie 11(5): 759-768
Huard, M.I.; Siebeling, R.J. 1971: Depressed cellular and humoral hemolysin responses of rabbits injected intraperitoneally with mineral oil before immunization Journal of Immunology 106(1): 266-270
Lyons, S.F.; Friedman, H. 1978: Cellular mechanisms of cholera toxin-mediated modulation of in vitro hemolysin formation by mouse immunocytes Journal of Immunology 120(2): 452-458
Kodama, H.; Moustafa, M.; Ishiguro, S.; Mikami, T.; Izawa, H. 1984: Extracellular virulence factors of fish Vibrio: relationships between toxic material, hemolysin, and proteolytic enzyme American Journal of Veterinary Research 45(10): 2203-2207
Herlax, V.; Bakas, L.S. 2002: Therapeutic applications of pore-forming lytic toxins: potential use of Escherichia coli alpha-hemolysin Medicina 62(1): 66-72