Accessibility of microsomal membrane proteins for protease K
Devichenskii, V.M.; Al'terman, M.A.; Zhikhareva, V.O.; Miasoedova, K.N.; Archakov, A.I.
Biokhimiia 44(4): 748-754
1979
ISSN/ISBN: 0320-9725 PMID: 435584 Document Number: 148250
The effect of protease K on the microsomal membrane proteins was studied. It was shown that treatment of the microsomal membranes by low concentrations of protease did not remove more than 35% of total membrane proteins. Treatment by higher protease concentrations removed about 50% of the proteins. The residual protein is accessible to protease after treatment of the membranes by phospholipase A2. A simultaneous treatment of the microsomal membrane by protease and phospholipase removed up to 80% of the membrane proteins. In this way about 30% of microsomal membrane proteins are protected by the phospholipid against the action of protease K.