Redistribution of Ca2+ ions in lymphocyte suspensions after treatment with an ionophore
Makarov, P.R.; Zinchenko, V.P.; Shartava, A.S.; Sakson, M.E.; Evtodienko, I.V.
Biofizika 29(6): 1006-1009
1984
ISSN/ISBN: 0006-3029 PMID: 6440593 Document Number: 223484
Ionophore A23187 induces three-phase kinetics of reduction of chlorotetracycline (CTC) lauded lymphocyte fluorescence: fast reduction, low increase and lower reduction of fluorescence. We suggested the following explanation of kinetics. The first phase is extinguishing of the Ca2+ - CTC complex fluorescence induced by ionophore A23187 perturbation of the cell membrane, the second one is the energy dependent Ca2+ entry into the cell and the third one is the slow efflux of Ca2+ ions from the cell to concentration gradient.