Redistribution of Ca2+ ions in Ehrlich ascites carcinoma cells under the action of deoxyglucose and inhibitors of the intracellular Ca2+- transporting system
Evtodienko, I.V.; Teplova, V.V.; Sidash, S.S.; Voĭtchak, L.
Biokhimiia 60(8): 1336-1343
1995
ISSN/ISBN: 0320-9725 PMID: 7578585 Document Number: 442220
Changes in free cytoplasmic Ca2+ ([Ca2+]i) in Ehrlich ascites tumour cells were studied under the effect of deoxyglucose and agents which modify transmembrane Ca2+ fluxes. It was shown that the reason for deoxyglucose-induced [Ca2+]i increase in Ca2+ release from internal stores and the influx from the external medium. Mitochondrial metabolic inhibitors (oligomycin+KCN, oligomycin++uncouplers of oxidative phosphorylation) induce themselves some rise in [Ca2+]i and increase the deoxyglucose effect on [Ca2+]i significantly. The conclusion was made that mitochondria can participate in cytosolic Ca2+ regulation and Ca2+ redistribution in tumour cells.