Effect of nitidine chloride on the life cycle of Ehrlich ascites carcinoma cells in mice
Fan, Y.J.; Zhou, J.; Li, M.
Zhongguo Yao Li Xue Bao 2(1): 46-49
1981
ISSN/ISBN: 0253-9756 PMID: 6461202 Document Number: 171781
The effect of nitidine chloride on the life cycle of Ehrlich ascites carcinoma (EAC) cells in CFW mice was studied by radioautography. The EAC cells were labeled on the 4th day after i.p. inoculation of 1 .times. 107 cells. The growth fraction was 0.86. When nitidine chloride 20 mg/kg was given i.p. the [3H] TdR pulse labeling index of EAC cells was unaffected within 26 h, but the mitotic index was reduced and the size of the cells increased, which suggested a G2 block. The cells in S phase might be killed by nitidine chloride 40 mg/kg. When the cells were labeled with [3H] TdR or [3H] UdR, the average labeling grain counts per cell revealed that the synthesis of DNA and RNA could be inhibited by nitidine chloride 20 mg/kg.