Hollow fiber immobilized L-asparaginase: in vivo and in vitro immunological studies
Callegaro, L.; Assone, F.; Cecconato, E.; Malinverni, A.; Pasteris, V.; Rossi, V.
International Journal of Artificial Organs 6(2): 91-96
1983
ISSN/ISBN: 0391-3988 PMID: 6840898 Document Number: 218084
The enzyme L-asparaginase was covalently immobilized on the inner surface of the hollow fibers utilized in a commercially available dialyzer by the periodate method. After sterilization with gamma radiation the bioreactor was able to metabolize in vivo, 90 per cent of circulating asparagine in two hours. The absence in blood of asparaginase-related protein fragments, released from the hollow fiber immobilized enzyme, was monitored using a specific enzyme-linked immunosorbent assay (ELISA).