Continuous production of carcinoembryonic antigen in hollow fiber cell culture units: brief communication
David, G.S.; Reisfeld, R.A.; Chino, T.H.
Journal of the National Cancer Institute 60(2): 303-306
1978
ISSN/ISBN: 0027-8874 PMID: 621750 Document Number: 132990
A line of cultured cells derived from a primary human adenocarcinoma of the colon was grown in the extracapillary spaces of hollow fiber tissue culture units in the absence of serum components of high molecular weight (> 10,000). The cells were capable of producing more than 30 .mu.g of carcinoembryonic antigen (CEA)/day, provided that the extracapillary fluid was changed frequently. The concanavalin A binding and molecular sieve chromatography properties of the tissue culture-derived CEA were similar to those of CEA isolated from metastatic colon cancer tissue.