Effect of total flavones of Sophora flavescens root on arrhythmia of cultured rat heart cells
Wang, J.Z.; Sun, F.L.; Han, H.W.; Zhang, B.H.; Yuan, J.S.; Ma, L.
Zhongguo Yao Li Xue Bao 4(1): 32-35
1983
ISSN/ISBN: 0253-9756 PMID: 6223495 Document Number: 218081
Total flavones (TF) extracted from the root of Sophora flavescens were dissolved in 1% Tween-80 (pH 7.8). The cardiomyocytes of newborn rats were cultured in Eagle MEM. TF 125-250 .mu.g/ml in the medium caused a decrease of 48 .+-. 19% of the beating rate of cell clusters. Some cultured myocytes showed spontaneous arrhythmias. When TF 125-250 .mu.g/ml were added into the medium, the arrhythmias of the cell clusters disappeared in 15/39 experiments (38%) and the flutter and fibrillation were markedly diminished in 19 experiments (49%). Flutter and fibrilation were seen in 20/25 experiments in ouabain 15-50 nM. These irregular beatings disappeared after addition of TF in 5/20 experiments and diminished conspicuously in 5/20 experiments. TF exerts a chronotropic and antiarrhythmic effect on cultured rat heart cells, but Tween-80 had no antiarrhythmic effect.