Selection of motile spermatozoa from human sperm by migration in follicular fluid
Arnal, F.; Humeau, C.; Hedon, B.; Catayee, G.
Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 177(1): 65-72
1983
ISSN/ISBN: 0037-9026 PMID: 6221781 Document Number: 217318
Using the property of human follicular fluid activation or "attraction" of human spermatozoa, a number of motile and active sperm was isolated from a sample of generally deficient sperm. The objective was to improve the success of artificial insemination of the conjoint. It was shown that it is possible to collect a much higher number of spermatozoa in the follicular fluid than in Menezzo's fluid. Taking into account the known toxicity of follicular fluid, the best results, based on number and motility were obtained with an mixture of follicular fluid and Menezzo's fluid in the ratio of 1:1.