One-step purification of L-type pyruvate kinase by microimmunoaffinity chromatography
Nürnberg, P.; Jacobasch, G.; Noll, F.; Thiele, B.
Biomedica Biochimica Acta 42(11-12): S283-S286
1983
ISSN/ISBN: 0232-766X PMID: 6675703 Document Number: 215745
L-type pyruvate kinase from several tissues was purified by a micro-method of immunaffinity chromatography and the subunit structure analysed by SDS-electrophoresis (1-3). Whereas in adult human kidney L as well as L' seem to be present in the normal erythrocyte only L' subunits could be identified.
Document emailed within 1 workday
Related Documents
Kechemir, D.; Max-Audit, I.; Calvin-Preval, M.C.; Rosa, R. 1986: Purification of human leucocyte pyruvate kinase Journal of Chromatography 383(1): 43-50Presek, P.; Glossmann, H.; Eigenbrodt, E.; Schoner, W.; Rübsamen, H.; Friis, R.R.; Bauer, H. 1980: Similarities between a phosphoprotein (pp60src)-associated protein kinase of Rous sarcoma virus and a cyclic adenosine 3':5'-monophosphate-independent protein kinase that phosphorylates pyruvate kinase type M2 Cancer Research 40(5): 1733-1741
Gumińska, M.; Wazewska-Czyzewska, M. 1980: Effect of Magnesium Diasporal on erythrocyte pyruvate kinase activity in healthy persons and in patients with pyruvate kinase deficiency Folia Medica Cracoviensia 22(3-4): 425-433
Singh, D.K.; Malhotra, S.P.; Singh, R. 1998: Purification and characterizaton of plastidic pyruvate kinase from developing seeds of Brassica campestris L Indian Journal of Biochemistry and Biophysics 35(6): 346-352
Głyda, A.; Kozak, W.; Waśko, R.; Horst-Sikorska, W.; Sawicka, J. 1994: Isolation and purification of transcortin (cortisol binding globulin--CBG) using two-step chromatography Ginekologia Polska 65(4): 180-184
Chen, S.H. 1993: Further study on the value of M2-type pyruvate kinase in the diagnosis of primary hepatocarcinoma Zhonghua Zhong Liu Za Zhi 15(4): 268-271
Cerwenka, H.; Aigner, R.; Bacher, H.; Werkgartner, G.; el-Shabrawi, A.; Quehenberger, F.; Mischinger, H.J. 1999: TUM2-PK (pyruvate kinase type tumor M2), CA19-9 and CEA in patients with benign, malignant and metastasizing pancreatic lesions Anticancer Research 19(1b): 849-851
Oremek, G.M.; Rutner, F.; Sapoutzis, N.; Sauer-Eppel, H. 2003: Tumor marker pyruvate kinase type tumor M2 in patients suffering from diabetic nephropathy Anticancer Research 23(2a): 1155-1158
Yang, L.; Jia, L.; Zou, H.; Kong, L.; Zhang, Y. 1997: Immobilized IDA-type Cu2+-chelating membrane affinity chromatography for purification of bovine liver catalase Se Pu 15(4): 292-295
Rybina, V.V. 1979: Increase in the amplitude of sulfhydryl group titer oscillations in solutions of creatine kinase and pyruvate kinase upon short-term illumination with infrared light Biofizika 24(6): 1093-1095
Shastri, Y.M.; Povse, N.; Schr, O.öder; Jürgen Stein 2008: Comparison of a Novel Fecal Marker- Fecal Tumor Pyruvate Kinase Type M2 (M2-PK) with Fecal Calprotectin in Patients with Inflammatory Bowel Disease: a Prospective Study Clinical Laboratory 54(9-10): 389-390
Tani, K. 1995: Pyruvate kinase Nihon Rinsho. Japanese Journal of Clinical Medicine 53 Su Pt 1: 369-372
Miwa, S. 1973: Pyruvate kinase deficiency Nihon Rinsho. Japanese Journal of Clinical Medicine 31(8): 2445-2449
Gumilńska, M.; Kedryna, T. 1974: Properties of neoplasm pyruvate kinase Folia Medica Cracoviensia 16(1): 128-129
Strumilo, S.A.; Senkevich, S.B.; Vinogradov, V.V. 1980: Purification of the pyruvate dehydrogenase complex from bovine adrenal cortex mitochondria Biokhimiia 45(5): 883-888
Standerfer, R.J.; Templeton, J.W.; Black, J.A. 1974: Anomalous pyruvate kinase deficiency in the Basenji dog American Journal of Veterinary Research 35(12): 1541-1543
Aitken, J.; Yudkin, J. 1970: Dietary carbohydrate, exercise and hepatic pyruvate kinase in the rat Proceedings of the Nutrition Society 29(1 Suppl): 7a
Whitney, K.M.; Lothrop, C.D. 1995: Genetic test for pyruvate kinase deficiency of Basenjis Journal of the American Veterinary Medical Association 207(7): 918-921
Whitney, K.M.; Lothrop, C.D. 1995: Genetic test for pyruvate kinase deficiency of Basenjis Journal of the American Veterinary Medical Association 207(7): 918-921
Jacobasch, G.; Holzhütter, H.G.; Gerth, C. 1983: Mathematical model of pyruvate kinase of chicken erythrocytes Biomedica Biochimica Acta 42(11-12): S289-S290