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Isolation and purification of transcortin (cortisol binding globulin--CBG) using two-step chromatography

Głyda, A.; Kozak, W.; Waśko, R.; Horst-Sikorska, W.; Sawicka, J.

Ginekologia Polska 65(4): 180-184

1994

ISSN/ISBN: 0017-0011
PMID: 7988920
Document Number: 427543
The isolation of cortisol and progesterone binding globulin (CBG) from pregnant women serum was performed using affinity and hydrophobic chromatography. The purity and specificity of isolated transcortin was tested by agarose gel electrophoresis using racket and cross immunoelectrophoresis and specific CBG antibodies. High purity and immunoreactivity of the isolated globulin destitute of other proteins contamination, were obtained.

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