Identification and quantitation of a metabolite of anethol dithiolthione in rat and mouse urine using high-performance liquid chromatography
Masoud, A.N.; Bueding, E.
Journal of Chromatography 276(1): 111-119
1983
ISSN/ISBN: 0021-9673 PMID: 6672005 Document Number: 212107
Urine samples from rats and mice fed anethol dithiolthione (ADT) [3-(p-methoxyphenyl)-1,2-dithiol-3-thione [an antidote for dryness of mouth produced by antidepressant drugs and a chemo- and radioprotective agent]] were analyzed using reversed-phase high-performance liquid chromatography. Urine was introduced directly on the liquid chromatograph which was modified by replacing the sample loop with a guard column. Highly polar urine components were washed off the guard column prior to chromatography. A major metabolite and the parent compound (ADT) were separated and detected using the chromatographic conditions described in this study. The metabolite was identified as desmethyl ADT. The identification was based on co-chromatography on 2 columns using 2 mobile phases and peak height ratios of the metabolite and the reference standard. Data pertaining to the pattern of excretion of ADT and desmethyl ADT in the animals studied are reported.