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Staphylococcal protein a and enhancement of disc RAST sensitivity

Oggel, J.D.; Dockhorn, R.J.

Annals of Allergy 50(3): 178-181

1983

ISSN/ISBN: 0003-4738
PMID: 6187247
Document Number: 205204
IgG has been suspected as an interesting factor in IgE-antigen specific RAST reaction. Such interference has been suggested by treatment of the patient's sera with staphylococcal protein A to reduce the concentration of IgG prior to carrying out the RAST measurements. This procedure includes incubation of killed staphylococcus organisms with patients' sera and noting the elevations in counts per minute of the RAST values to various antigens. This evaluation was carried out in 11 patients. It would appear that modification of "blocking" IgG antibody might increase RAST sensitivity.

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