Enhancement of platelet sensitivity to ADP-aggregation by isometric exercise in arteriosclerosis patients and its prevention
Sano, T.; Motomiya, T.; Yamazaki, H.; Shimamoto, T.
Thrombosis and Haemostasis 37(2): 329-338
1977
ISSN/ISBN: 0340-6245 PMID: 195363 Document Number: 119996
A new method for assessment of platelet sensitivity to ADP-aggregation was devised. Its reproducibility and the correlations between the values obtained by this method, the optical density (OD) method and the screen filtration pressure (SFP) method were assessed. This method can be performed without centrifugation, avoiding mechanical stress to platelets, using only 0.8 ml of blood and inexpensive equipment. It may reflect different aspects of platelet function from the OD method and the SFP method, despite the positive significant correlations between the values obtained by these 3 methods. It was highly reproducible and is thought to be clinically useful. By using this method the effect of sustained isometric exercise by handgripping on platelet aggregability was assessed in coronary sclerotic and cerebral arteriosclerotic patients on placebo and EG-626 , a newly synthesized cyclic AMP phosphodiesterase inhibitor. On placebo an enhancement of platelet sensitivity was observed after isometric exercise in coronary and cerebral arteriosclerotic patients, but not in healthy control subjects. The enhancement was prevented by pretreatment of EG-626, administered orally 1.5 h prior to exercise.