Use of the immunoperoxidase method on paraffin sections
Babaev, V.R.; Shchetnikova, L.A.
Arkhiv Patologii 45(1): 76-78
1983
ISSN/ISBN: 0004-1955 PMID: 6342576 Document Number: 202351
Using an indirect immunoperoxidase technique, the methods of fixation and treatment of paraffin sections were explored for subsequent identification of myosin of smooth and skeletal muscle cells, actin, filamin, and immunoglobulins. Bouin's fluid and ethanol were found to be the most adequate fixators for immunomorphological analysis. Pronase treatment of paraffin sections increased the intensity of specific reactions considerably. Incubation of paraffin sections with normal goat serum and hydrogen peroxide decreased nonspecific staining of the sections.