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Immunoperoxidase localization of treponema pallidum: its use in formaldehyde-fixed and paraffin-embedded tissue sections

Beckett, J.H.; Bigbee, J.W.

Archives of Pathology and Laboratory Medicine 103(3): 135-138

1979

ISSN/ISBN: 0003-9985
PMID: 85440
Document Number: 146933
The spirochete, Treponema pallidum, the causative agent of syphilis, has been successfully localized in formaldehydefixed and paraffin-embedded tissue sections using rabbit anti-T pallidum antiserum with two immunoperoxidase techniques. These techniques, the indirect peroxidase-labeled antibody method and the peroxidase-antiperoxidase (PAP) method are compared for sensitivity and degree of nonspecific staining. Both offer substantial advantages over conventional silver-impregnation techniques, but the indirect peroxidase-labeled antibody method seems better, based on the intensity of staining and the simplicity of procedure.

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Kim, D.H.; Hong, Y.K.; Egholm, M.; Strauss, W.M. 2001: Non-disruptive PNA-FISH protocol for formalin-fixed and paraffin-embedded tissue sections Biotechniques 31(3): 472 475-6
Wunderlin, E.; Pospischil, A. 1992: Peroxidase-antiperoxidase labelling of Leishmania amastigotes in formalin fixed and paraffin embedded tissue sections Berliner und Münchener Tierärztliche Wochenschrift 105(9): 302-304
Bjerkås, I.; Landsverk, T. 1986: Identification of Toxoplasma gondii and Encephalitozoon cuniculi by immunoperoxidase techniques and electron microscopy, in stored, formalin-fixed, paraffin-embedded tissue Acta Veterinaria Scandinavica 27(1): 11-22
Tanimoto, T.; Ohtsuki, Y. 1996: Evaluation of antibodies reactive with porcine lymphocytes and lymphoma cells in formalin-fixed, paraffin-embedded, antigen-retrieved tissue sections American Journal of Veterinary Research 57(6): 853-859
Lasota, J.; Jasinski, M.; Debiec-Rychter, M.; Szadowska, A.; Limon, J.; Miettinen, M. 1998: Detection of the SYT-SSX fusion transcripts in formaldehyde-fixed, paraffin-embedded tissue: a reverse transcription polymerase chain reaction amplification assay useful in the diagnosis of synovial sarcoma Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology Inc 11(7): 626-633
Trost, T.H. 1984: Immunologic diagnosis of cutaneous lymphomas. I. a new immunoperoxidase technic for the demonstration of cell- and tissue-bound antigens in formalin-fixed paraffin-embedded tissues using protein A-peroxidase as a universal second antibody for mono- and polyclonal antibodies Zeitschrift für Hautkrankheiten 59(14): 929-934
Kemény, E.; Iványi, B.; Németh, A.; Sonkodi, S. 1983: Application of the immunoperoxidase technique to formalin fixed, paraffin embedded kidney biopsies Zentralblatt für Allgemeine Pathologie U. Pathologische Anatomie 128(1-2): 119-126
Ikeda, S. 2015: Development of a Dual Detection Method with Fluorescence In Situ Hybridization and Immunostaining on Formalin-Fixed Paraffin-Embedded Tissue Sections--Molecular Pathological Detection Techniques and Their Applications to Pathological Diagnosis Rinsho Byori. Japanese Journal of Clinical Pathology 63(6): 769-773
Miettinen, M. 1992: CD30 distribution. Immunohistochemical study on formaldehyde-fixed, paraffin-embedded Hodgkin's and non-Hodgkin's lymphomas Archives of Pathology and Laboratory Medicine 116(11): 1197-1201
Dervan, P.A.; Magee, H.M.; Buckley, C.; Carney, D.N. 1992: Proliferating cell nuclear antigen counts in formalin-fixed paraffin-embedded tissue correlate with Ki-67 in fresh tissue American Journal of Clinical Pathology 97(5 Suppl 1: S21-S28
Nakanishi, T.; Ito, M.; Ueda, K.; Wada, S.; Fujioka, S.; Tsuji, M.; Takubo, T. 2012: Clinical applications of MALDI imaging using sliced sections of formalin-fixed paraffin-embedded tissues and longitudinal sliced hairs Rinsho Byori. Japanese Journal of Clinical Pathology 60(2): 125-130
Gorbacheva, I.V.; Golitsyna, E.P.; Varshavskiĭ, V.A.; Pal'tseva, E.M. 2011: Modification of direct immunofluorescence assay to study paraffin-embedded renal and skin tissue sections Arkhiv Patologii 73(1): 49-51
Chewawiwat, N.; Lulitanond, V.; Nimmanahaeminda, K.; Thamprasert, K.; Nicomrat, D.; Ponglikitmongkol, M. 1995: Use of PCR-PIRA for screening of a point mutation at codon 12 in K-ras oncogene obtained from paraffin embedded tissue sections Southeast Asian Journal of Tropical Medicine and Public Health 26(Suppl 1): 329-332
Mahaisavariya, P.; Chaiprasert, A.; Manonukul, J.; Khemngern, S.; Tingtoy, N. 2005: Detection and identification of Mycobacterium species by polymerase chain reaction (PCR) from paraffin-embedded tissue compare to AFB staining in pathological sections Journal of the Medical Association of Thailand 88(1): 108-113
Gast, A.; Shachar, D.; Kotik, A.; Kugel, C.; Bublil, N. 2019: Identification of Comingled Tissue Within a Formalin-Fixed Paraffin-Embedded Sample Using Forensic Genetics - a Tool for Prevention of Misdiagnosis Harefuah 158(12): 778-782
Moura, H.; Schwartz, D.A.; Bornay-Llinares, F.; Sodré, F.C.; Wallace, S.; Visvesvara, G.S. 1997: A new and improved "quick-hot Gram-chromotrope" technique that differentially stains microsporidian spores in clinical samples, including paraffin-embedded tissue sections Archives of Pathology and Laboratory Medicine 121(8): 888-893
Tamura, M.; Fukaya, T.; Murakami, T.; Uehara, S.; Yajima, A. 1998: Analysis of clonality in human endometriotic cysts based on evaluation of X chromosome inactivation in archival formalin-fixed, paraffin-embedded tissue Laboratory Investigation; A Journal of Technical Methods and Pathology 78(2): 213-218
Osaki, M.; Adachi, H.; Gomyo, Y.; Yoshida, H.; Ito, H. 1997: Detection of mycobacterial DNA in formalin-fixed, paraffin-embedded tissue specimens by duplex polymerase chain reaction: application to histopathologic diagnosis Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology Inc 10(1): 78-83
Babaev, V.R.; Shchetnikova, L.A. 1983: Use of the immunoperoxidase method on paraffin sections Arkhiv Patologii 45(1): 76-78
Randhawa, P.S.; Jaffe, R.; Faruki, H.; Rinaldo, C.R.; Ho, M.; Demetris, A.J.; Manez, R.; Kusne, S.; Ehrlich, G.D. 1994: Detection of cytomegalovirus in formalin-fixed paraffin-embedded donor, native and allograft liver tissue using a multiplex polymerase chain reaction-liquid hybridization assay Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology Inc 7(1): 125-128