Application of the immunoperoxidase technique to formalin fixed, paraffin embedded kidney biopsies
Kemény, E.; Iványi, B.; Németh, A.; Sonkodi, S.
Zentralblatt für Allgemeine Pathologie U. Pathologische Anatomie 128(1-2): 119-126
1983
ISSN/ISBN: 0044-4030 PMID: 6356703 Document Number: 213410
Different immunohistological methods were compared in 30 renal biopsy cases. The direct immunoperoxidase method gave identical results with immunofluorescence in 88% concerning IgG and C3, while IgA, IgM and fibrinogen have been identically detected by the PAP method in 82%. The non-identical results of the direct method consisted, with one exception, of negative readings, while the non-identical PAP readings were positive with two exceptions. Thus the trypsin-immunoperoxidase method proved to be well applicable on paraffin embedded formalin fixed material. In 10 cases the HRP-labelled direct method was applied also to fresh, frozen sections. It gave almost entirely identical results to immunofluorescence.