Modification of direct immunofluorescence assay to study paraffin-embedded renal and skin tissue sections
Gorbacheva, I.V.; Golitsyna, E.P.; Varshavskiĭ, V.A.; Pal'tseva, E.M.
Arkhiv Patologii 73(1): 49-51
2011
ISSN/ISBN: 0004-1955 PMID: 21506343 Document Number: 648513
Immunofluorescence assay has been widely used so far to diagnose glomerular and some skin diseases. The optimal antigen persistence is achieved using the frozen sections; however, their considerable shortcoming is the impossibility to long store preparations and to use a morphology archive. Our laboratory has modified a direct immunofluorescence study on paraffin-embedded renal and skin tissue sections, substantially increasing its accessibility.
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