Autoradiographic detection of tritiated choline chloride in brain tissue. A method of microscopic detection in paraffin sections
Rummelfänger, H.; Fröhlich, J.
Journal für Hirnforschung 19(4): 291-294
1978
ISSN/ISBN: 0021-8359 PMID: 739137 Document Number: 136682
It is presented a autoradiographic method for microscopical detection of 3H-choline chloride activity after intraventricular application in freeze-dried and paraffin-embedded brain sections. The 10 micrometer thick sections are treated with diethyl ether and than exposed during 90 days above calcium chloride at 4 degree C using the sandwich technique (with stripping film). After separate film explanation and section staining the film and the brain sections are mounted together.
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