Effect of chloroquine on different stages of asexual forms of Plasmodium falciparum in culture
Zhou, Y.C.; Guan, W.B.; Huang, W.J.; Pan, W.Q.
Zhongguo Yao Li Xue Bao 4(2): 137-140
1983
ISSN/ISBN: 0253-9756 PMID: 6225305 Document Number: 200868
FCC-1/HN strain of P. faciparum in culture was synchronized with 5% mannitol solution in particular stages to be tested. Each well of plastic plates was filled with 200 .mu.l of culture medium containing 5 .mu.l of infected and uninfected RBC of G1, G3, G5 and their abnormal parasite rate of the parasites after exposure to CQ 32 ng/ml for 8 h were 16.9, 23.2, 37.7 ng/ml and 93.1%, 16.2%, 3.4%, respectively. The ED50 of G2, G4, G6, and their abnormal parasite rate after exposure to CQ 16 ng/ml for 48 h were 11.2, 16.4, 17.9 ng/ml and 81.8%, 3%, 0%, respectively. Apparently, the efficacy of CQ was more remarkable at R stage than at T stage, which was more remarkable than at S stage.