Liquid-chromatographic determination of amitryptyline and its metabolites in serum, with adsorption onto glass minimized
Edelbroek, P.M.; de Haas, E.J.; de Wolff, F.A.
Clinical Chemistry 28(10): 2143-2148
1982
ISSN/ISBN: 0009-9147 PMID: 7127744 Document Number: 193635
To study correlations between the concentrations, in serum, of amitriptyline and its most important metabolites with clincial response in patients, high-performance liquid chromatographic method or routine determination of serum into hexane/isoamyl alcohol (99/1 by vol). Perazine is the internal standard. To minimize irreversible adsorption of the drugs onto the glassware, 5 .mu.g of maprotiline is added to the organic phase just before evaporation. After a 10-min resolution on a silica column eluted with acetonitrile/methanol/NH4OH (1 mol/l), absorbance is measured at 240 nm. Only chlorimipramine, doxepin, procainamide and N-acetylprocainamide may interfere with assay of the compounds that probably are therapeutically relevant: amitriptyline, nortriptyline and E-10-hydroxynortriptyline. Uremia, lipemia and icterus also do not affect the analysis.